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Ben Reeves' second-half strike ended Dover's interest in the FA Cup for another season after a valiant display against League 1 MK Dons.
The home faithful booed their side off at half-time but were cheering five minutes after the restart when Reeves broke the deadlock, firing home from close range.
The outcome was no disgrace for Chris Kinnear's side who battled to the end in front of more than 1,000 travelling fans.
Skrill South Dover, playing a 4-1-4-1 formation, worked hard in the first half against a home side looking short on confidence after a poor run.
Chris Kinnear Jnr blasted over from 20 yards and home goalkeeper David Martin punched clear off the head of Nathan Elder as Dover looked to get on the scoresheet in the first half.
Home boss Karl Robinson sent on substitute George Baldock at half-time and he lifted the home side, going close twice in the second half.
Following Reeves' goal, Sean Raggett headed against his own post as Dover came under pressure for the first time in the game.
Raggett then went close at the other end late on, heading over the bar, after Chelsea loanee Patrick Bamford had blazed over with four minutes left for MK Dons.
Subs Richard Orlu and Michael Bakare also went close for Dover, but the visitors gained no reward for a spirited, gutsy display.