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Dartford lost 3-2 at home to League 1 side Leyton Orient on Friday night.
Lee Noble and trialist Tom Derry scored the goals for Tony Burman's side.
On Saturday, Joe Healy earned Welling United a 1-1 draw against Gillingham after Antonio German had put the Gills ahead. Read Craig Tucker's report from Park View Road.
A goal from trialist Jake Reid saw Dover Athletic beat Margate 1-0 at Crabble.
Ebbsfleet United were 4-0 winners against Dagenham & Redbridge, with Charlie Sheringham scoring twice. Billy Bricknell and Alex Osborn were also on target as the Fleet put their League 2 visitors to the sword.
Here is Steve Tervet's match report.
Pierre Joseph-Dubois netted for Bromley in their 2-1 home defeat to a Millwall XI.
Maidstone United drew 1-1 with Concord Rangers at the Gallagher Stadium, Jack Harris scoring for the Stones, while a Harry Harding penalty saw Tonbridge Angels beat Eastbourne Borough 1-0.
Ian Draycott scored both Folkestone Invicta goals in their 2-1 win away to Chatham Town, a Josh Vincent own goal having given the home side the lead.
At Reachfields, Hythe Town beat Canterbury City 4-1. Jordan Wells scored twice for Tim Dixon's side and there were also goals for Josh Burchell and Ryan Philpott.
Whitstable Town were beaten 2-1 at The Belmont by AFC Hornchurch despite a goal from Adam Woollcott and Herne Bay lost to Woodstock Sports by the same scoreline in the Carl Davis Memorial Trophy. Having returned from his trial with Maidstone United, Jordan Casey scored for Bay.
Ramsgate drew 1-1 with Ashford United, Sittingbourne won 2-1 at Thamesmead Town and Cray Wanderers ran out 2-1 winners away to Tooting & Mitcham United.